Neuronal differentiation through GPI-anchor cleavage
نویسندگان
چکیده
منابع مشابه
GLUT4-containing vesicles are released from membranes by phospholipase D cleavage of a GPI anchor.
We have previously developed a cell-free assay from rat skeletal muscle that displayed in vitro glucose transporter 4 (GLUT4) transfer from large to small membrane structures by the addition of a cytosolic protein fraction. By combining protein fractionation and the in vitro GLUT4 transfer assay, we have purified a glycosylphosphatidylinositol (GPI) phospholipase D (PLD) that induces transfer o...
متن کاملEnzymatic mechanism of GPI anchor attachment clarified
A group of eukaryotic cell surface proteins are anchored to the outer leaflet of the plasma membrane by glycosylphosphatidylinositol (GPi). in human cells, about 150 GPi-anchored proteins of various functions, such as hydrolytic enzymes, receptors, protease inhibitors, adhesion molecules, and complement regulatory proteins, have been known, and more will be added to the list. GPi is a glycolipi...
متن کاملGPI-anchor and GPI-anchored protein expression in PMM2-CDG patients
BACKGROUND Mutations in PMM2 impair phosphomannomutase-2 activity and cause the most frequent congenital disorder of glycosylation, PMM2-CDG. Mannose-1-phosphate, that is deficient in this disorder, is also implicated in the biosynthesis of glycosylphosphatidyl inositol (GPI) anchors. OBJECTIVE To evaluate whether GPI-anchor and GPI-anchored proteins are defective in PMM2-CDG patients. METH...
متن کاملGPI anchor transamidase of Trypanosoma brucei: in vitro assay of the recombinant protein and VSG anchor exchange.
GPI8 from Trypanosoma brucei was cloned and expressed in Escherichia coli. TbGPI8 encodes a 37 kDa protein (35 kDa after removal of the putative signal sequence) with a pI of 5.5. It contains one potential N-glycosylation site near the N-terminus but no C-terminal hydrophobic region. Enzyme activity assays using trypanosomal lysates or recombinant TbGpi8 exhibited cleavage of the synthetic pept...
متن کاملLeukemia inhibitory factor inhibits neuronal terminal differentiation through STAT3 activation.
The discovery of stem cells in the adult central nervous system raises questions concerning the neurotrophic factors that regulate postnatal neuronal development. Olfactory receptor neurons (ORNs) are a useful model, because they are capable of robust neurogenesis throughout adulthood. We have investigated the role of leukemia inhibitory factor (LIF) in postnatal neuronal development by using O...
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ژورنال
عنوان ژورنال: Cell Cycle
سال: 2017
ISSN: 1538-4101,1551-4005
DOI: 10.1080/15384101.2016.1259894